Journal: Oncology Letters
Article Title: Impact of fibroblast activation protein on osteosarcoma cell lines in vitro
doi: 10.3892/ol.2014.1788
Figure Lengend Snippet: Cellular function tests of FAP in osteosarcoma cell lines. (A and B) Knockdown of FAP significantly reduced cell growth. (C and D) In vitro cell matrix adhesion assays were performed to assess the effect of FAP on the adhesiveness of osteosarcoma cells. (E and F) Knockdown of FAP significantly reduced cell adhesion, detected using an electric cell-substrate impedance sensing model system. FAP, fibroblast activation protein.
Article Snippet: The 9600 model of the ECIS instrument (Applied BioPhysics, Inc., Troy, NY, USA) was used for attachment (adhesion) using a 96W1E plate, as well as a motility assay (wounding assay) ( – ).
Techniques: Cell Function Assay, Knockdown, In Vitro, Electric Cell-substrate Impedance Sensing, Activation Assay